Cell Line Information:

Designations: 293 [HEK-293]Depositors: FL GrahamGrowth Properties: adherentOrganism: Homo sapiensMorphology: epithelialSource:Organ: embryonic kidneyApplications: efficacy testing, transfection host, viruscide testingReceptors: vitronectinTumorigenic: YESAge: fetusComments: The cells containes Adenovirus 5 DNA from left ends of the viral genome. The line is excellent for titrating human adenoviruses. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit. The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2)

The Transfection Reagent:

293 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on 293 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for 293 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of 293 Cell Avalanche™ Transfection Reagent for transfection on 293 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

Amini M, Wang H, Belkacemi A, Jung M, Bertl A, etal.Identification of Inhibitory Ca²⁺ Binding Sites in the Upper Vestibule of the Yeast Vacuolar TRP Channel. iScience. 2019 Jan 25;11:1-12. PubMed PMID: 30572205; PubMed Central PMCID: PMC6299153.

Features:

• Specifically optimized to deliver nucleic acids into 293 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Simplest and straightforward protocols
• Deliver single or multiple plasmids
• Compatible with serum
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems