Cell Information:

Human Follicle Outer Root Sheath Cells (HORSC) are primary cells isolated from the outer root sheath of the hair follicle. Outer root sheath cells can be cultured in vitro to form epidermal equivalents, and can be used for autologous grafting. Furthermore, HORSC are reported to support stem cells in the bulge region of the outer sheath. These bulge cells are mutlipotent, critical for the self-renewal of hair follicles, and also have the capacity to regenerate sebaceous glands and epidermis.

The Transfection Reagent:

Transfection of primary cells, such as HORSC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Follicle Outer Root Sheath Cell (HORSC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HORSC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HORSC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Follicle Outer Root Sheath Cell (HORSC) Avalanche™ Transfection Reagent for transfection on HORSC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in HORSC.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Compatible with serum
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems