Cell line Information:

Designations:293T/17 [HEK 293T/17]Depositors: Rockefeller Univ.Growth Properties: adherentOrganism: Homo sapiens Morphology: epithelialSource: kidneyAntigen Expression: SV40 T antigen [45408]Age: fetusComments: The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17. These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.

The Transfection Reagent:

293T/17 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 293T/17 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 293T/17 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into 293T/17 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Simplest and straightforward protocols
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate format