Cell Line Information:

Designations: 3197-3Depositors: JE DeLarcoGrowth Properties: adherentOrganism: Mus musculusMorphology: fibroblastOrgan: embryoCell Type: fibroblastAge: embryoThe cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus. They release a growth factor in serum free medium. They do not produce virus particles. The cells have a tendency to slough off the flask surface. Tested and found negative for ectromelia virus (mousepox).

The Transfection Reagent:

3197-3 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on 3197-3 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for 3197-3 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of 3197-3 Cell Avalanche™ Transfection Reagent for transfection on 3197-3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in 3197-3 Cells.
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems