Cell Line Information:

Designations: 786-OOrganism: Homo sapiens (human)Tissue: renal cell adenocarcinoma; kidneyAge: 58 yearsGender: maleEthnicity: CaucasianMorphology: epithelialGrowth properties: adherentTumorigenic: yes, in immunosuppressed hamstersDepositors: R.D. WilliamsThis line was derived from a primary clear cell adenocarcinoma. The cells display both microvilli and desmosomes, and can be grown in soft agar. The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH, has PTH like activity.

786-O is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

786-O Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on 786-O Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for 786-O Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of 786-O Cell Avalanche™ Transfection Reagent for transfection on 786-O Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in 786-O Cells.
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Compatible with serum
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed