Cell Line Information:

Designations: A-431Depositors: DJ Giard, SA AaronsonGrowth Properties: adherentOrganism: Homo sapiensMorphology: epithelialSource: skinTissue: epidermisDisease: epidermoid carcinomaApplications: transfection hostTumorigenic: YesAge: 85 yearsGender:femaleComments: The epidermoid carcinoma cell line A-431, derived from an 85-year-old female, is one of a series of cell lines established from solid tumors by D.J. Giard, et al.

The Transfection Reagent:

A-431 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on A-431 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for A-431 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of A-431 Cell Avalanche™ Transfection Reagent for transfection on A-431 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

references:

Wang S, Allen N, Vickers TA, Revenko AS, Sun H, et al. Cellular uptake mediated by epidermal growth factor receptor facilitates the intracellular activity of phosphorothioate-modified antisense oligonucleotides. Nucleic Acids Res. 2018 Mar 5;PubMed PMID: 29514240; PubMed Central PMCID: PMC5909429.

Features:

• Unique formulation-maximize transfection performance in A-431 Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Compatible with serum
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems