Cell Line Information:

Designations: A549Depositors: M LieberGrowth Properties: adherentOrganism: Homo sapiensMorphology: epithelialOrgan: lungDisease: carcinomaIsolation: Isolation date: 1972Applications: transfection hostIsoenzymes: G6PD, BAge: 58 yearsGender: maleEthnicity: CaucasianThis line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. Further studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway. The cells are positive for keratin by immunoperoxidase staining.

A549 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

A549 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting A549 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for A549 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in A549 Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems