Cell LineInformation:

Designations: ARPE-19Organism: Homo sapiens (human)Tissue: eye; retinal pigmented epithelium; retinaAge: 19 yearsGender: maleMorphology: epithelialGrowth properties: adherentKaryotype: diploidAntigen Exp: RPE-specific markers CRALBP and RPE-65Depositors: L.M. HjelmelandARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident. These cells form stable monolayers, which exhibit morphological and functional polarity. ARPE-19 expresses the RPE-specific markers CRALBP and RPE-65.

The Transfection Reagent:

ARPE-19 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on ARPE-19 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for ARPE-19 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of ARPE-19 Cell Avalanche™ Transfection Reagent for transfection on ARPE-19 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into ARPE-19 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems