Cell Information:

Beyond the role of the bladder epithelium in forming a barrier, this tissue may play an unappreciated but important role in water, salt, and solute homeostasis. One of the most exciting functions of the bladder epithelium is its potential role as a sensory transducer.Urothelial carcinoma of the bladder is unique among epithelial carcinomas in its divergent pathways of tumorigenesis. Low-grade papillary tumors rarely become muscle-invasive and they frequently harbour gene mutations that constitutively activate the receptor tyrosine kinase–Ras pathway. By contrast, most high-grade invasive tumors progress to life-threatening metastases and have defects in the p53 and the retinoblastoma protein pathways.Human bladder epithelial cells (HBlEpC) can be used as a powerful tool in studying not only bladder epithelial development and function, but also the processes by which epithelial cells achieve and maintain their specialized plasma membrane domains, and how these functions are disrupted in disease.

The Transfection Reagent:

Transfection of primary cells, such as HBlEpC, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Bladder Epithelial Cell (HBlEpC) Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on HBlEpC. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for HBlEpC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Bladder Epithelial Cell (HBlEpC) Avalanche® Transfection Reagent for transfection on HBlEpC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HBlEpC
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Compatible with transfection in any plate formats
• Developed and manufactured by EZ Biosystems