Cell Information:

The cervix consists of ectocervix and the endocervix, and contains two types of cells, columnar and squamous epithelial cells. Columnar epithelial cells form a single layer of mucous-secreting cells located in the endocervix and a variable portion of the ectocervix while squamous epithelial cells are located on the ectocervix. The transformation zone is the region on the ectocervix where over time the more fragile columnar epithelial cells are replaced with more durable squamous epithelial cells. This area is particularly vulnerable to pre-cancerous changes (dysplasia) due to the high turnover rate and low maturation level of the cells.

Human Cervical Epithelial Cells (HCvEpCs)are a useful tool for studying various aspects of pathology and biology of the human cervical epithelium in vitro.

The Transfection Reagent:

Transfection of primary cells, such as HCvEpC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Cervical Epithelial Cell (HCvEpC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HCvEpC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HCvEpC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in HCvEpC.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems