Cell Information:

Although lymphatic micorvascular endothelial cells have many properties in common with blood microvascular endothelial cells, they also have unique functions due to their structural features. Lymphatic capillaries have thin and discontinuous base membrane and are directly connected to the insterstitial collagens by elastic anchoring filaments. The endothelial cells of lymphatic vessel wall loosely overlap to form easily opened minivalves that make them highly permeable. Recent studies demonstrate that tumor lymphangiogenesis is a major component of the metastatic process and members of the VEGF family are key mediators of lymphangiogenesis in tumors.Human Dermal Lymphatic Microvascular Endothelial (HDLMVEC) are usually isolated from lymphatic capillaries of normal human dermis. HDLMVEC could be used for the following applications:

• Dermal microvasculature pathology
• Inflammation
• Oncology
• Diabetes
• Pulmonary disease/disorders
• Drug toxcity

The Transfection Reagent:

Transfection of primary cells, such as HDLMVEC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Dermal Lymphatic Microvascular Endothelial Cell (HDLMVEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HDLMVEC. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for HDLMVEC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HDLMVEC
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems