Cell Information:

Dermal Microvascular Endothelial Cells (HMVEC/CADMEC) usually are isolated from normal human neonatal foreskin or adult skin capillaries. They are responsive to cytokine stimulation in the expression of cell adhesion molecules.HMVEC express CD36 constitutively, while large vessel endothelial cells do not.HMVEC is an organ-specific endothelial cell system for the study of angiogenesis, tumor metastasis, wound healing, inflammation, macromolecule transport, and drug metabolism. There is growing evidence of differences in physiology and pharmacology between microvascular endothelial cells and macrovascular endothelial cells.

Applications:

• Dermal microvasculature pathology
• Inflammation
• Oncology
• Diabetes
• Pulmonary disease/disorders
• Drug toxcity

The Transfection Reagent:

Transfection of primary cells, such as HMVEC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Dermal Microvascular Endothelial Cell (HMVEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HMVEC. The proprietary formulation oflipids and polymers ensures the highest possible transfection efficiencies and viabilities for HMVEC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HMVEC
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems