Cell Information:

Human Fibroblast-like synoviocytes (HFLS) represent a specialised cell type located inside joints in the synovium. HFLS are long known for their role in joint destruction by producing cytokines and metalloproteinases that contribute to cartilage degradation. Proinflammatory cytokines induce the proliferation, collagenase and aggrecanase production and GM-CSF secretion on HFLS. Ongoing arthritis research also has shown that HFLS express apoptosis and P53 mutations

Human Fibroblast-Like Synoviocytes (HFLS) are usually isolated from normal synovial tissues. These cells can be used as a good in vitro model for studyingthe pathogenesis of chronic inflammatory diseases, such as rheumatoid arthritis.

The Transfection Reagent:

Transfection of primary cells, such as HFLS, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Fibroblast-Like Synoviocyte (HFLS, HFLS-OA, HFLS-RA) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HFLS. The proprietary formulation oflipids and polymers ensures the highest possible transfection efficiencies and viabilities for HFLS. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Fibroblast-Like Synoviocyte (HFLS, HFLS-OA, HFLS-RA) Avalanche™ Transfection Reagent for transfection on HFLS. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems