Cell Information:

Human Lung Microvascular Endothelial Cells (HLMVEC) and elevated expression of chemoattractant cytokines are critical for directed migration of leucocytes into the lung during the induction and perpetuation of acute lung injury. HLMVEC provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro.

Applications:

• Vascular pathology
• Inflammation
• Oncology
• Pulmonary hypertension
• Pulmonary disease/disorders
• Drug toxcity

The Transfection Reagent:

Transfection of primary cells, such as HLMVEC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HLMVEC. The proprietary formulation oflipids and polymers ensures the highest possible transfection efficiencies and viabilities for HLMVEC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HLMVEC
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems