Cell Information:

Human Mammary Epithelial Cells (HMEpC) are usually derived from normal adult mammary glands. It was shown that normal HMEpC have a high amount of cyclin-dependent kinase 6 activity, which restrains HMEpC proliferation, and loss or down-regulation of cdk 6 may play a role in breast tumor development1. Transforming HMEpC to immortality and malignancy was a useful model for studying early breast carcinogenesis. Autocrine colony-stimulating factor receptors may contribute to invasive progression in epithelial cancer was demonstrated in a 3-dimensional culture of HMEpC.

Applications:

• Breast cancer
• Cellular function and differentiation
• Physiology
• Toxicology
• Hormone regulation

The Transfection Reagent:

Transfection of primary cells, such as HMEpC, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Mammary Epithelial Cell (HMEpC, HMEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HMEpC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HMEpC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HMEpC
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems