Cell Information:

Mesenchymal stem cells (hMSC) are usually harvested and cultured from normal human bone marrow.The cells have the ability to differentiate into osteogenic, chondrogenic and adipogenic lineages. Cells are positive for CD105, CD166, CD29, and CD44. Cells test negative for CD14, CD34 and CD45.The potential of hMSC to maintain multipotency and proliferate extensively in vitro provides new avenues for cell-based therapy in the restoration of damaged or diseased tissue. Recent reports indicate that hMSC are capable of cell fates crossing germ layer boundaries. In addition to differentiating into multi-lineages of the mesoderm, these cells can also differentiate into neurons of ectodermal origin and hepatocyte-like cells of endodermal origin.

The Transfection Reagent:

Transfection of primary cells and stem cells, such as hMSC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Mesenchymal Stem Cell (Human Marrow Stromal Cell) (hMSC) Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting hMSC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for hMSC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in hMSC.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems